soal pilihan ganda dan pembahasan trigonometri kelas 11 pdf

Differences between the values obtained in two independent runs were â‰¤0.3 log IU for the plasmid and â‰¤0.6 log IU/mg for the reference patient, showing a high inter-run reproducibility. A good linear trend between the expected values and those generated by the assay was observed at different concentrations of both plasmid and reference patient (R 2 Â =Â 0.994 and 0.962, respectively). The linearity and reproducibility of the modified protocol was tested by quantifying serial dilutions of a full-length HBV containing plasmid and it-HBV DNA from a reference patient. To quantitate it-HBV DNA in a series of 70 liver biopsies obtained from hepatitis B chronic patients, a modified version of the COBAS Â® Ampliprep/COBAS Â® TaqMan HBV test v2.0 was used for this purpose. Intrahepatic total HBV DNA (it-HBV DNA) level might reflect the size of virus reservoir and correlate with the histological status of the liver.

Salpini, Romina Piermatteo, Lorenzo Gill, Upkar Battisti, Arianna Stazi, Francesca Guenci, Tania Giannella, Sara Serafini, Valentina Kennedy, Patrick T F Perno, Carlo Federico Svicher, Valentina Ciotti, Marco Quantification of intrahepatic total HBV DNA in liver biopsies of HBV-infected patients by a modified version of COBASÂ® Ampliprep/COBASÂ®TaqMan HBV test v2.0. Combination of the Total Nucleic Acid Isolation Kit on the COBAS AmpliPrep instrument with the LightCycler Parvovirus B19 Quantification Kit provides a reliable and time-saving tool for sensitive and accurate detection of parvovirus B19 DNA. Identification of a persistent parvovirus B19-infected individual by the polymerase chain reaction among 51 anti-parvovirus B19 IgM-negative samples underlines the importance of additional nucleic acid testing in pregnancy and its superiority to serology in identifying the risk of parvovirus B19 transmission via blood or blood products. Relative sensitivity and specificity in routine samples from pregnant women are 100% and 93%, respectively. The lower detection limit is 234 IU/ml at a 95% hit-rate, linear range approximately 104-1010 IU/ml, and overall precision 16 to 40%. The kit combination processes 72 samples per 8-hour shift.

Nucleic acids were extracted using the Total Nucleic Acid Isolation Kit (Roche Diagnostics) and amplified with the LightCycler Parvovirus B19 Quantification Kit. We report the analytical performance of the LightCycler Parvovirus B19 Quantification Kit (Roche Diagnostics) using nucleic acids isolated with the COBAS AmpliPrep instrument.

The COBAS AmpliPrep instrument (Roche Diagnostics GmbH, D-68305 Mannheim, Germany) automates the entire sample preparation process of nucleic acid isolation from serum or plasma for polymerase chain reaction analysis. Schorling, Stefan Schalasta, Gunnar Enders, Gisela Zauke, Michael Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR

Soal pilihan ganda dan pembahasan trigonometri kelas 11 pdf